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PRESERVATION OF POLYMORPHONUCLEAR NEUTROPHILS AT CRYOGENIC TEMPERATURES 193 K (-80 C).

Author(s) : YAMASHITA T.

Type of article: Article

Summary

THE PRESERVATION OF POLYMORPHONUCLEAR NEUTROPHILS (PMNS) AT CRYOGENIC TEMPERATURES 193 K (-80 C) WAS STUDIED. A CONSIDERABLE DIFFERENCE AMONG CRYOPROTECTIVE AGENTS WAS OBSERVED IN THEIR PROTECTIVE EFFECT AGAINST FREEZE INJURY OF NEUTROPHILS. WHEN PMNS WERE FROZEN IN ME2SO ALONE, THE ABILITY OF PMNS TO EXCLUDE DYE WAS RETAINED AFTER 3 DAYS OF CRYOPRESERVATION, WHILE CHEMOTAXIS WAS INHIBED MARKEDLY. ONE-WEEK PRESERVATION PRODUCED THE DEATH OF 50% OF THE CELLS. TO IMPROVE THE PROTECTIVE EFFECT OF ME2SO ADDITIVES SUCH AS GLUCOSE, ATP, AND ALBUMIN WERE INCLUDED IN THE FREEZING MEDIUM. ADDITION OF ALBUMIN DISPLAYED THE MOST DISTINCT IMPROVEMENT IN THE RECOVERY OF CHEMOTAXIS. A COMBINATION OF ME2 AND ATP MARKEDLY PROTECTED PHAGOCYTOSIS AND ADHESION FROM FREEZE INJURY.

Details

  • Original title: PRESERVATION OF POLYMORPHONUCLEAR NEUTROPHILS AT CRYOGENIC TEMPERATURES 193 K (-80 C).
  • Record ID : 1981-0281
  • Languages: English
  • Source: Cryobiology - vol. 17 - n. 2
  • Publication date: 1980/04

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