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MORPHOLOGY OF CHLAMYDIA ORGANISMS AS REVEALED BY FREEZE-SUBSTITUTION FIXATION TECHNIQUE.

Author(s) : UEHIRA K., MATSUMOTO A., SUDA T.

Type of article: Article

Summary

THE PAPER DESCRIBES THE TECHNIQUE BY WHICH CHLAMYDIA PSITTACI ORGANISMS WERE FROZEN AT LIQUID NITROGEN TEMPERATURE THEN TREATED WITH OSMIUM TETROXIDE, 4%, IN ACETONE AT 194 K (-79 DEG C). COMPARABLE SPECIMENS WERE TREATED WITH OSMIUM TETROXIDE, 1%, AND DEHYDRATED IN ETHANOL. WITH THE FREEZE-SUBSTITUTION FIXATION METHOD THE CYTOPLASMIC MEMBRANE WAS WELL PRESERVED AND SURFACE PROJECTIONS WERE READILY SEEN. THEY WERE NOT SEEN IN THE SPECIMENS TREATED BY THE OTHER METHOD. C.R.F.

Details

  • Original title: MORPHOLOGY OF CHLAMYDIA ORGANISMS AS REVEALED BY FREEZE-SUBSTITUTION FIXATION TECHNIQUE.
  • Record ID : 1984-2111
  • Languages: English
  • Source: Kawasaki med. J. - vol. 9 - n. 2
  • Publication date: 1983
  • Document available for consultation in the library of the IIR headquarters only.

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