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PARTICLE DISPLACEMENT IN EPITHELIAL CELL MEMBRANES OF RAT PROSTATE AND PANCREAS INDUCED BY ROUTINE LOW TEMPERATURE FIXATION.

Author(s) : KACHAR B.

Type of article: Article

Summary

WHEN CARRIED OUT AT 277 K (4.C) IN CONTRAST TO 310 K (37.C), GLUTARALDEHYDE FIXATION FOR ROUTINE FREEZE-FRACTURE SPECIMEN PREPARATION RESULTS IN DISPLACEMENT OF MEMBRANE PARTICLES OF EPITHELIAL CELLS OF RAT VENTRAL PROSTATE. PARTICLE-FREE AREAS ARE OBSERVED IN ENDOPLASMIC RETICULUM (ER), NUCLEAR ENVELOPE, AND GOLGI MEMBRANES ; PARTICLE AGGREGATION IS OBSERVED IN LATERAL AND BASAL PORTIONS OF THE PLASMA MEMBRANE. MULTILAMELLAR ARRANGEMENT OF LIPIDS CAN BE OBSERVED IN SOME OF THE LARGE PARTICLE-FREE AREAS. THIN SECTION OBSERVATION INDICATES THAT IN THE ER, PARTICLE-FREE AREAS COEXIST WITH SEGREGATION OF CYTOPLASMIC GROUND SUBSTANCE FROM THE MEMBRANE SURFACES. PARTICLE-FREE PATCHES ARE ALSO OBSERVED IN THE ER MEMBRANES OF PANCREATIC TISSUES FIXED AT 277 K.

Details

  • Original title: PARTICLE DISPLACEMENT IN EPITHELIAL CELL MEMBRANES OF RAT PROSTATE AND PANCREAS INDUCED BY ROUTINE LOW TEMPERATURE FIXATION.
  • Record ID : 1982-0295
  • Languages: English
  • Source: Cell. Biol. int. Rep. - vol. 4 - n. 4
  • Publication date: 1980
  • Document available for consultation in the library of the IIR headquarters only.

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