ShareRemoval of the zona pellucida and parthenogenetic activation affect rates of survival of ultrarapidly frozen mouse oocytes.
Author(s) : LAI A. C. H., RYAN J. P., SAUNDERS D. M.
Type of article: Article
Summary
Survival rates on thawing were assessed for ultrarapidly frozen mouse oocytes following the removal of zonae pellucidae using an acid Tyrode's solution, pronase or a mechanical dissection technique. The results support the hypothesis that increased survival of ultrarapidly frozen pronuclear stage embryos is the result of changes to the plasma membrane and/or to the zona pellucida that occur following fertilization.
Details
- Original title: Removal of the zona pellucida and parthenogenetic activation affect rates of survival of ultrarapidly frozen mouse oocytes.
- Record ID : 1996-2564
- Languages: English
- Source: Reprod. Fertil. Dev. - vol. 6 - n. 6
- Publication date: 1994
- Document available for consultation in the library of the IIR headquarters only.
Links
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Ovule; Cryoprotectant; Cryobiology; DMSO; Survival; Mouse
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