SharePRESERVATION OF MOUSE EMBRYOS BY TWO-STEP FREEZING.
Author(s) : WOOD M. J., FARRANT J.
Type of article: Article
Summary
EIGHT-CELL MOUSE EMBRYOS IN 1.5 M DMSO WERE PRESERVED IN LN2 BY A TWO STEP PROCEDURE. FIFTEEN MINUTES EXPOSURE AT 253 K (-20 DEG C) PROTECTED THE EMBRYOS AGAINST DAMAGE DURING RAPID COOLING TO 77 K (-196 DEG C) AND DURING RAPID WARMING AND RAPID DILUTION. SINCE SURVIVAL WAS POOR ON SLOW WARMING IT IS SUGGESTED THAT THE METHOD PERMITS THE FORMATION OF SOME INTRACELLULAR ICE.
Details
- Original title: PRESERVATION OF MOUSE EMBRYOS BY TWO-STEP FREEZING.
- Record ID : 1981-0318
- Languages: English
- Source: Cryobiology - vol. 17 - n. 2
- Publication date: 1980/04
Links
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryoprotectant; DMSO; Survival; Mouse; Embryo; Cryopreservation
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- Date : 1991
- Languages : English
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- Author(s) : SHAW J. M., TROUNSON A. O.
- Date : 1989
- Languages : English
- Source: Cryobiology - vol. 26 - n. 5
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LOW TEMPERATURE PRESERVATION OF 2-CELL MOUSE EM...
- Author(s) : MEZEVIKINA L. M., BEREZOVSKAJA O. P., VEPRINCEV B. N.
- Date : 1988
- Languages : Russian
- Source: Kriobiologija - n. 3
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DEPRESSION OF THE ICE-NUCLEATION TEMPERATURE OF...
- Author(s) : RALL W. F.
- Date : 1983
- Languages : English
- Source: Biophys. J. - vol. 41 - n. 1
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The use of sodium hyaluronate in freezing media...
- Author(s) : PALASZ A., ALKEMADE S., MAPLETOFT R. J.
- Date : 1993/04
- Languages : English
- Source: Cryobiology - vol. 30 - n. 2
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