Research on enzyme-labeled methamidophos cryopreservation for enzyme thermal sensor.
[In Chinese. / En chinois.]
Author(s) : NGUYEN V. L., XU F., YU J., et al.
Type of article: Article
Summary
Cryopreservation of enzyme-labeled methamidophos was studied using the composite protective agent (2.0mol/L NaCl, 4.0mol/L xylitol and 1.25mol/L glycerol) obtained from accelerated test. The results show that the relationship between enzyme activity loss rate and storage temperature meet Arrhenius equation. After 6 months, the retention rate of enzyme activity is 7.13% (without protective agents) and 97.44% (with protective agents) under the storage condition of – 18°C. Comparing to theoretical prediction value according to chemical kinetics, the relative standard deviation was 3.10% (without protective agents) and 0.51% (with protective agents), respectively. The result will lay the foundation for the development of thermal pesticide residue biosensor.
Available documents
Format PDF
Pages: 51-56
Available
Public price
20 €
Member price*
15 €
* Best rate depending on membership category (see the detailed benefits of individual and corporate memberships).
Details
- Original title: [In Chinese. / En chinois.]
- Record ID : 30004210
- Languages: Chinese
- Source: Journal of Refrigeration - vol. 33 - n. 143
- Publication date: 2012/02
Links
See other articles in this issue (11)
See the source
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Sensor; Pesticide; Enzyme; Cryopreservation
-
Effect of cryoprotectants on activity of select...
- Author(s) : ADAM M. M., RANA K. J., MCANDREW B. J.
- Date : 1995/02
- Languages : English
- Source: Cryobiology - vol. 32 - n. 1
View record
-
THE MECHANISM OF CRYOPROTECTION OF PROTEINS BY ...
- Author(s) : CARPENTER J. F., CROWE J. H.
- Date : 1988
- Languages : English
- Source: Cryobiology - vol. 25 - n. 3
View record
-
CRYOPRESERVATION OF SKIN USING A MURINE MODEL: ...
- Author(s) : KEARNEY J. N., WHELDON L. A., GOWLAND G.
- Date : 1990
- Languages : English
- Source: Cryobiology - vol. 27 - n. 1
View record
-
IMPROVEMENT OF THE FREEZING PROCESS OF ENDOCRIN...
- Author(s) : OVCAROVA G. P.
- Date : 1985
- Languages : Russian
- Source: Kholodilnaya Tekhnika - n. 1
View record
-
A method for the cryopreservation of liver pare...
- Author(s) : DIENER B., UTESCH D., BEER N., DÜRK H., OESCH F.
- Date : 1993/04
- Languages : English
- Source: Cryobiology - vol. 30 - n. 2
View record