Research on enzyme-labeled methamidophos cryopreservation for enzyme thermal sensor.

[In Chinese. / En chinois.]

Author(s) : NGUYEN V. L., XU F., YU J., et al.

Type of article: Article

Summary

Cryopreservation of enzyme-labeled methamidophos was studied using the composite protective agent (2.0mol/L NaCl, 4.0mol/L xylitol and 1.25mol/L glycerol) obtained from accelerated test. The results show that the relationship between enzyme activity loss rate and storage temperature meet Arrhenius equation. After 6 months, the retention rate of enzyme activity is 7.13% (without protective agents) and 97.44% (with protective agents) under the storage condition of – 18°C. Comparing to theoretical prediction value according to chemical kinetics, the relative standard deviation was 3.10% (without protective agents) and 0.51% (with protective agents), respectively. The result will lay the foundation for the development of thermal pesticide residue biosensor.

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Pages: 51-56

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Details

  • Original title: [In Chinese. / En chinois.]
  • Record ID : 30004210
  • Languages: Chinese
  • Source: Journal of Refrigeration - vol. 33 - n. 143
  • Publication date: 2012/02

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