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Research on ultrasound-induced nucleation to improve cryoablation effectiveness.

Author(s) : LI W., YANG X., WANG Y., LIU B., YOUNAS S.

Type of article: IJR article

Summary

This research proposes a novel combination therapy that utilizes ultrasound-assisted cryoablation to enhance the lethality of cryoablation in Hepatocellular Carcinoma (HepG2) cells. Ultrasound is used to induce ice nucleation, followed by thawing at a set temperature. The efficacy of this approach was evaluated by assessing cell survival rates in hepatocellular carcinoma cells using Acridine Orange/ Propidium Iodide (AO/PI) staining and the Cell Counting Kit-8 (CCK-8), comparing ultrasound-assisted cryoablation with cryoablation alone. At a cooling rate of 10 °C/min and lower nucleation temperatures, a significant reduction in cell survival rate was observed (15.60 ± 2.60 %). Cryomicroscopic observations revealed that enhanced intracellular and extracellular ice formation led to increased cellular damage and higher cryoablation lethality. This innovative therapy provides a promising approach for localized treatment and highlights the potential of cryoablation devices incorporating ultrasound-induced nucleation.

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Pages: 489-499

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Details

  • Original title: Research on ultrasound-induced nucleation to improve cryoablation effectiveness.
  • Record ID : 30033494
  • Languages: English
  • Source: International Journal of Refrigeration - Revue Internationale du Froid - vol. 170
  • Publication date: 2025/02
  • DOI: http://dx.doi.org/10.1016/j.ijrefrig.2024.12.011

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