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SHORT TERM STORAGE OF MOUSE EMBRYOS BY NON-FREEZING METHODS.

Author(s) : SUN Z. Q., CRAVALHO E. G.

Summary

SHORT-TERM STORAGE OF EMBRYOS IS USEFUL IN SOME PARTICULAR CIRCUMSTANCES SUCH AS EMBRYO TRANSPORTATION, TRANSPLANTATION AND EMBRYOLOGICAL RESEARCH. ALTHOUGH THE ACTUAL MECHANISMS OF EMBRYO SURVIVAL RATE REDUCTION AFTER FREEZE-THAW CRYOPRESERVATION ARE NOT COMPLETELY UNDERSTOOD, IT IS BELIEVED THAT ONE OF THE MAIN CAUSES OF DAMAGE IS THE RESULT OF ICE FORMATION. NON-FREEZING LOW TEMPERATURE PRESERVATION COULD BE A WAY FOR EMBRYO SHORT-TERM STORAGE. IN THE PRESENT WORK, A SMALL INCUBATOR WITH A COMPUTER-CONTROLLED REFRIGERATOR AND HEATER SYSTEM WAS DEVELOPED. 579 MOUSE EMBRYOS AT DIFFERENT STAGES OF DEVELOPMENT HAD BEEN CYCLED BETWEE NNFEN RT THE FACTORS WHICH INFLUENCED THE SURVIVAL DURING STORAGE WERE OPTIMIZED. MOUSE EMBRYOS CAN BE STORED AND REMAIN VIABLE FOR PERIODS UP TO SEVEN DAYS.

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Pages: 31-36

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Details

  • Original title: SHORT TERM STORAGE OF MOUSE EMBRYOS BY NON-FREEZING METHODS.
  • Record ID : 1988-1264
  • Languages: English
  • Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
  • Publication date: 1987/08/24
  • Document available for consultation in the library of the IIR headquarters only.

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