Spermatogenic evaluation, cryogenic preservation and fertility of the "pacu", Piaractus mesopotamicus (Holmberg, 1887), sperm, from induced spawning.
[In Portuguese. / En portugais.]
Author(s) : FOGLI DA SILVEIRA W., KAVAMOTO E. T., GESTAROLLI M. A., GODINHO H. M., RAMOS S. M., SILVEIRA A. N.
Type of article: Article
Summary
In order to develop artificial reproduction in commercial freshwater fish, the seminal characteristics, cryogenic preservation and fertility of "pacu" sperm were studied. The sperm was frozen in liquid nitrogen vapour and the cryogenic preservation was carried out in special container. After 24 hours of freezing, the sperm was thawed and under microscopy examination showed 20% live spermatozoa. After one year of cryopreservation, the fertilization test revealed 21.07% of fertilized eggs, whereas for the control fresh semen the fertility was 34.55%. Therefore, sperm cryopreservation of the "pacu" and its utilization in the artificial reproduction routine is feasible.
Details
- Original title: [In Portuguese. / En portugais.]
- Record ID : 1994-3886
- Languages: Portuguese
- Source: Bol. Inst. Pesca - vol. 17
- Publication date: 1990
- Document available for consultation in the library of the IIR headquarters only.
Links
See the source
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Thawing; Sperm; Cryobiology; Survival; Fish; Cryopreservation; Freezing
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