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Sterile application of the open pulled straw (OPS) vitrification method.

Author(s) : VAJTA G., LEWIS I. M., KUWAYAMA M., GREVE T., CALLESEN H.

Type of article: Article

Summary

This report describes an attempt to overcome sterile conditions for the recently introduced OPS vitrification method. The whole vitrification procedure is performed in a laminar hood using filtered liquid nitrogen for cooling. The embryo-containing OPS is then placed into a 0.5 ml plastic straw pre-cooled in the vapour of liquid nitrogen. The straw is hermetically sealed and stored in non-filtered liquid nitrogen. At warming, the large straw is kept vertically and half-immersed in the liquid nitrogen, the end cut and then the OPS is quickly removed and warmed directly in the culture medium. The procedure is fairly simple, safe and does not decrease the in vitro survival rates of in vitro produced bovine blastocysts. The possibility to perform sterile OPS vitrification is a prerequisite for using this technique in accordance with the sanitary regulations and may result in a significant extension of its application.

Details

  • Original title: Sterile application of the open pulled straw (OPS) vitrification method.
  • Record ID : 1999-3878
  • Languages: English
  • Source: Cryo-Letters - vol. 19 - n. 6
  • Publication date: 1998/11
  • Document available for consultation in the library of the IIR headquarters only.

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