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Cryopreservation of rat islets of Langerhans: a comparison of two techniques.

Author(s) : RICH S. J., SWIFT S., THIRDBOROUGH S. M., RUMFORD G., JAMES R. F. L., LONDON N. J. M.

Type of article: Article

Summary

The paper compares two methods with identical equilibration protocols: one with cooling at 0.25 deg C/min to -40 deg C prior to transferring to liquid nitrogen and subsequent warming at 200 deg C/min and sucrose dilution of DMSO (method A) and the other with cooling at 0.3 deg C/min to -70 deg C prior to transferring to liquid nitrogen and warming at 50 deg C/min followed by stepwise dilution of DMSO (method B). Seven hundred fifty fresh islets in the size range 100-200 micrometer consistently reversed diabetes, whereas 1000 method A cryopreserved islets and 2000 method B cryopreserved islets were required. Cryopreservation by either method resulted in impaired islet function compared to that of fresh islets, although this functional loss could be partially compensated by the transplantation of greater numbers of cryopreserved islets.

Details

  • Original title: Cryopreservation of rat islets of Langerhans: a comparison of two techniques.
  • Record ID : 1994-1898
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 4
  • Publication date: 1993/08

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