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SURVIVAL OF GRANULOCYTE (GM-CFC) AND ERYTHROBLAST (CFUE AND BFUE ) PRECURSORS IN NORMAL HUMAN MARROW FROZEN BY A SIMPLE TWO-STAGE METHOD: PRELIMINARY RESULTS.

ETUDE DE LA SURVIE DES PRECURSEURS GRANULOCYTAIRES (GM-CFC) ET ERYTHROBLASTIQUES (CFUE ET BFUE ) DE MOELLE HUMAINE NORMALE CONGELEE PAR UNE TECHNIQUE SIMPLE EN DEUX ETAPES : RESULTATS PRELIMINAIRES.

Author(s) : BERTHIER R.

Type of article: Article

Summary

DESCRIPTION OF A TWO-STAGE FREEZING PROCEDURE FOR HUMAN BONE MARROW, OF WHICH A CELL SUSPENSION IS PLACED IN 2-ML POLYPROPYLENE TUBES PLUNGED 20 MIN IN BATHS AT A TEMPERATURE ADJUSTED TO 245, 241, 236 OR 232 K (-28, -32, -37 OR -41 DEG C). THE TUBES ARE THEN DIRECTLY PLUNGED IN LIQUID NITROGEN AND REMAIN THERE 30 MIN. THAWING IS QUICK WATER-BATH AT 315 K (42 DEG C). FOR GRANULOCYTE PRECURSORS, THE OPTIMUM FREEZING TEMPERATURE SEEMS TO BE 236 K. THE TESTS SHOULD BE FURTHERED FOR ERYTHROBLAST PRECURSORS, BUT THEY SEEM TO BE ENCOURAGING. G.G.

Details

  • Original title: ETUDE DE LA SURVIE DES PRECURSEURS GRANULOCYTAIRES (GM-CFC) ET ERYTHROBLASTIQUES (CFUE ET BFUE ) DE MOELLE HUMAINE NORMALE CONGELEE PAR UNE TECHNIQUE SIMPLE EN DEUX ETAPES : RESULTATS PRELIMINAIRES.
  • Record ID : 1983-2389
  • Languages: French
  • Source: Nouv. Rev. fr. Hématol. - vol. 24 - n. 4
  • Publication date: 1982
  • Document available for consultation in the library of the IIR headquarters only.

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