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FACTORS INFLUENCING MYELOID STEM CELL (CFU-C) SURVIVAL AFTER CRYOPRESERVATION OF HUMAN MARROW AND CHRONIC GRANULOCYTIC LEUKEMIA CELLS.

Author(s) : MA D. D. F., JOHNSON L. A., CHAN P. M.

Type of article: Article

Summary

THE OPTIMAL COOLING RATE FOR THE HUMAN MYELOID STEM CELLS (CFU-C) RANGED FROM 1 TO 3K PER MINUTE. THE MYELOID STEM CELLS (CFU-C) MAINTAINED THEIR VIABILITY FOR UP TO ONE YEAR OF STORAGE IN LIQUID NITROGEN, AFTER AN INITIAL 20% REDUCTION DUE TO THE FREEZING PROCEDURE. MYELOID STEM CELLS SURVIVED BETTER WHEN THAWED AND DILUTED AT ROOM TEMPERATURE (RT) THAN AT 277 K (4 DEG C). THE VIABILITY OF THAWED STEM CELLS DECREASED WHEN STORED AT RT FOR MORE THAN 1 HR.

Details

  • Original title: FACTORS INFLUENCING MYELOID STEM CELL (CFU-C) SURVIVAL AFTER CRYOPRESERVATION OF HUMAN MARROW AND CHRONIC GRANULOCYTIC LEUKEMIA CELLS.
  • Record ID : 1982-2110
  • Languages: English
  • Source: Cryobiology - vol. 19 - n. 1
  • Publication date: 1982

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