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TENTATIVE UNDERSTANDING OF THE MECHANISM OF HEMOGLOBIN OXIDATION DURING FREEZE-DRYING WITHOUT A PROTECTANT.

ESSAI DE COMPREHENSION DU MECANISME DE L'OXYDATION DE L'HEMOGLOBINE PENDANT LA LYOPHILISATION EN L'ABSENCE DE PROTECTEUR.

Author(s) : LABRUDE P., CHAILLOT B., VIGNERON C.

Summary

THE FREEZE-DRYING OF OXYHEMOGLOBIN RESULTS IN THE FORMATION OF ABOUT 50% METHEMOGLOBIN. THE AUTHORS STUDY THE POSSIBLE EFFECTS OF THE CONCENTRATION AND THE PH OF THE SOLUTIONS TO BE FREEZE-DRIED. FOR CONCENTRATIONS RANGING BETWEEN 10 AND 200 G/L, THE METHEMOGLOBINE RATIO REMAINS ABOUT CONSTANT. THERE IS A SIGNIFICANT CORRELATION BETWEEN THE PH AND SATURATION. DURING DRYING, DENATURATION (BROWNING) OCCURS QUITE EARLY: THE APPARATUS BEING STOPPED WHEN THE PRODUCT TO BE FREEZE-DRIED IS AT -40 DEG C, METHEMOGLOBIN REACHES THERE ALREADY 10% IMMEDIATELY AFTER THAWING. ITS RATIO INCREASES RAPIDLY DURING THE REWARMING OF THE PRODUCT ABOUT 40% WHEN STOPPING AT -30 DEG C.

Details

  • Original title: ESSAI DE COMPREHENSION DU MECANISME DE L'OXYDATION DE L'HEMOGLOBINE PENDANT LA LYOPHILISATION EN L'ABSENCE DE PROTECTEUR.
  • Record ID : 1983-2421
  • Languages: French
  • Publication date: 1982/07/08
  • Source: Source: Congr. natl. Transfus. sang., Saint-Etienne
    n. 12.

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