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The cryopreservation of liposomes. 2. Effect of particle size on crystallization behaviour and marker retention.

Author(s) : TALSMA H., STEENBERGEN M. J. van, CROMMELIN D. J. A.

Type of article: Article

Summary

Liposome dispersions were frozen in a differential scanning calorimeter. In the cooling curves of the dispersions a heat flow below -40 deg C is observed. This heat flow is due to the crystallization of maximally supercooled water. Evidence is provided that at this temperature, part or all of the encapsulated water in the liposomes crystallizes. At a cooling rate of 10 K/min only for small liposomes with particle sizes below approximately 0.2 micrometer the internal volume crystallizes at -40 deg C. After a freezing/thawing cycle of the liposomal dispersions retention of the water-soluble marker carboxyfluorescein was studied in different conditions (size of liposomes, temperature of freezing and temperature of storage after freezing and before thawing).

Details

  • Original title: The cryopreservation of liposomes. 2. Effect of particle size on crystallization behaviour and marker retention.
  • Record ID : 1993-0534
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 1
  • Publication date: 1992/02

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