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Subzero osmotic characteristics of intact and disaggregated hepatocyte spheroids.

Author(s) : KORNISKI B., DARR T. B., HUBEL A.

Type of article: Article

Summary

This study has been conducted to examine basic transport characteristics of pig hepatocytes cultured as spheroids for use in a bioartificial liver. Static osmotic experiments were conducted by subjecting hepatocyte spheroids in solutions of increasing sucrose concentrations. The technique of freeze substitution was used to examine the structure inside the spheroid during freezing. At a low cooling rate of 1 °C per minute, increasing amounts of intercellular ice formed between the cells. At a higher cooling rate of 100 °C per minute small intracellular ice crystals formed. This study shows the location of ice in a freezing hepatocyte spheroid and confirms that the cells cultured as spheroids do not transport water in the same manner as isolated cells.

Details

  • Original title: Subzero osmotic characteristics of intact and disaggregated hepatocyte spheroids.
  • Record ID : 2000-2234
  • Languages: English
  • Source: Cryobiology - vol. 38 - n. 4
  • Publication date: 1999/06

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