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Testing of cryopreserved concentrates at infusion leads to standardization of engraftment in autologous peripheral blood progenitor cell transplantation.

Number: pap. ID: 806

Author(s) : MERICKA P., STRAKOVÁ H., LÁNSKÁ M., et al.

Summary

The aim of the study performed in 29 patients undergoing autologous periheral blood progenitor cell (PBPC) transplantation was to obtain data on total number of infused nucleated cells and on their viability as well as on the number of infused CD 34+ cells and CFU-GM. To prevent delayed engraftment (more than 21 days) the minimum cell dose was adjusted. As the PBPC concentrates were cryopreserved in presence of 10% dimethylsulphoxide (DMSO) the consequences of this approach on the total infused volume were evaluated. The results showed high post-thaw viability of infused nucleated and/or mononuclear cells. Engraftment within 21 days was achieved in 97% of patiens, the only case of slightly delayed engraftment (on day 23) was associated with a CFU-GM dose lower than 2 x 10(5)/kg of the patient´s weight while the CD 34+ cell dose was normal. The effort to control the risk of delayed engraftment led, however, to the need to accept higher risk of the DMSO toxicity to prevent exceeding of the recommended DMSO daily dose (1 ml/1 kg of the patient´s weight). Repeated infusions in two or more days were necessary in 43% of patients.

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Details

  • Original title: Testing of cryopreserved concentrates at infusion leads to standardization of engraftment in autologous peripheral blood progenitor cell transplantation.
  • Record ID : 30001892
  • Languages: English
  • Source: Proceedings of the 23rd IIR International Congress of Refrigeration: Prague, Czech Republic, August 21-26, 2011. Overarching theme: Refrigeration for Sustainable Development.
  • Publication date: 2011/08/21

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