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THE EFFECT OF THE COOLING RATE OF FRESH SEMEN ON THE LABORATORY QUALITY OF CRYOPRESERVED SPERMATOZOA.

[In Czech. / En tchèque.]

Author(s) : SKROBANEK P., LEDEC M.

Type of article: Article

Summary

IMMEDIATELY AFTER COLLECTION, THE SEMEN OF THE WHITE BROAD-BREASTED TURKEY WAS COOLED TO 278K (5 DEG C) AT THE RATE OF 8, 20, 40, 68, 88, 100, 124 AND 140 K PER MIN. IT WAS DILUTED AT THE RATIO OF 1: 4 WITH A CRYOPRESERVATION DILUENT CONTAINING ETHYLENE GLYCOL (8%). IT WAS FROZEN IN LIQUID NITROGEN VAPOURS FOR 3 MIN BEFORE STORAGE AT 77 K (-196 DEG C). PERCENTAGE OF LIVE SPERMATOZOA AND PERCENTAGE OF MORPHOLOGICALLY NORMAL SPERMATOZOA WERE EVALUATED AFTER THAWING. THE COOLING RATES OF 8 AND 20 K PER MIN WERE THE MOST EFFECTIVE.

Details

  • Original title: [In Czech. / En tchèque.]
  • Record ID : 1985-2688
  • Languages: Czech
  • Source: Zivoc. Vyroba - vol. 28 - n. 8
  • Publication date: 1983
  • Document available for consultation in the library of the IIR headquarters only.

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