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The influence of cooling rate on survival of frozen cells differs in monolayers and in suspensions.

Author(s) : ARMITAGE W. J., JUSS B. K.

Type of article: Article

Summary

Cells were cooled to -80 deg C at rates ranging from 0.2 to 10 deg C/minute in the presence of 10% (v/v) cryoprotectant (DMSO, propane-1, 2-diol or glycerol). After freezing and thawing, there was a very strong influence of cooling rate on cell survival in monolayers. Survival was highest (higher than 80%) after cooling at 0.2 deg C/minute, but declined sharply to lower than 40% after cooling at 1 deg C/minute. Conversely, isolated cells showed high survival at 1 deg C/minute but lower survival at 0.2 deg C/minute: indeed cell survival after cooling at 0.2 deg C/minute was lower in suspensions than in monolayers.

Details

  • Original title: The influence of cooling rate on survival of frozen cells differs in monolayers and in suspensions.
  • Record ID : 1997-2469
  • Languages: English
  • Source: Cryo-Letters - vol. 17 - n. 4
  • Publication date: 1996/07
  • Document available for consultation in the library of the IIR headquarters only.

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