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PRELIMINARY INVESTIGATION OF OSMOTIC PROPERTIES AND FREEZING BEHAVIOUR OF HUMAN ENDOTHELIAL CELLS.

Author(s) : SCHAFER A. T.

Type of article: Article

Summary

CULTURED ENDOTHELIAL CELLS FROM HUMAN UMBILICAL VEINS WERE TRYPSINATED, WASHED, AND RESUSPENDED IN PHOSPHATE BUFFERED SALINE. THE BOYLE-VAN'T HOFF PLOT INDICATING THEIR OSMOTIC RESPONSE IN HYPERTONIC SALINE YIELDED AN OSMOTICALLY INACTIVE VOLUME FRACTION OF 42.9%. BY MEANS OF A FLUORESCENCE ASSAY, THE VIABILITY AS A FUNCTION OF COOLING RATE WAS EVALUATED IN THE RANGE BETWEEN 1 AND 400 K/MIN (AT A CONSTANT WARMING RATE OF 300 K/MIN), YIELDING A MAXIMUM VIABILITY OF ABOUT 35% BETWEEN 3 AND 5 K/MIN. THE ADDITION OF 10% GLYCEROL EXTENDS THE RANGE OF FAVORABLE COOLING RATES TO ABOUT 1 TO 10 K/MIN AND INCREASES THE MAXIMUM VIABILITY TO MORE THAN 40%.

Details

  • Original title: PRELIMINARY INVESTIGATION OF OSMOTIC PROPERTIES AND FREEZING BEHAVIOUR OF HUMAN ENDOTHELIAL CELLS.
  • Record ID : 1986-2106
  • Languages: English
  • Source: Cryo-Letters - vol. 7 - n. 1
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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