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THE INFLUENCE OF CRYOPRESERVATION ON THE ULTRASTRUCTURAL MORPHOLOGY OF HUMAN THYROID CELLS.

Author(s) : SHILOH H.

Type of article: Article

Summary

THE PURPOSE WAS TO INVESTIGATE THE EFFECTS OF THE FREEZE-THAW PROCEDURE ON THE ULTRASTRUCTURAL FEATURES OF HUMAN THYROID CELLS. 4 DIFFERENT STAGES WERE COMPARED: (1) FRESH SURGICAL TISSUE, SERVING AS CONTROL, (2) CELL SUSPENSION BEFORE FREEZING, (3) CELL SUSPENSION AFTER THAWING, AND (4) MONOLAYER CELL CULTURE, OBTAINED FROM CELLS AFTER THAWING. ELECTRON MICROSCOPIC EXAMINATION SHOWED THAT THE FREEZE-THAW PROCEDURE USED CAUSED ONLY MINOR INTRACELLULAR ALTERATIONS RESTRICTED TO MITOCHONDRIA. THESE ULTRASTRUCTURAL CHANGES WERE NOT PARALLELED BY A DECREASE IN THE VITALITY.

Details

  • Original title: THE INFLUENCE OF CRYOPRESERVATION ON THE ULTRASTRUCTURAL MORPHOLOGY OF HUMAN THYROID CELLS.
  • Record ID : 1989-0353
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 4
  • Publication date: 1987

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