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THE INFLUENCE OF TEMPERATURE ON THE FUNCTION OF RENAL CORTICAL SLICES FROZEN IN VARIOUS CRYOPROTECTANTS.

Author(s) : CLARK P., HAWKINS H. E., KAROW A. M. Jr

Type of article: Article

Summary

RENAL CORTICAL SLICES WERE FROZEN TO VARIOUS SUBZERO TEMPERATURES AFTER TREATMENT WITH 2.1 M OF ONE OF THREE CRYOPROTECTANTS: DMSO, ETHYLENE GLYCOL, OR GLYCEROL. THE EFFECTS OF COOLING ON TISSUE (K+)/(NA+) WERE TESTED BY HOLDING THE SLICES AT EACH TEMPERATURE FOR APPROPRIATE PERIODS PRIOR TO REWARMING. THE TOXICITY OBSERVED AT HIGHER SUBZERO TEMPERATURES IS EXPECTED TO BE OF IMPORTANCE, PARTICULARLY FOR SLOWLY COOLED TISSUES WHICH ARE EXPOSED TO THESE TEMPERATURES FOR RELATIVELY LONG PERIODS OF TIME.

Details

  • Original title: THE INFLUENCE OF TEMPERATURE ON THE FUNCTION OF RENAL CORTICAL SLICES FROZEN IN VARIOUS CRYOPROTECTANTS.
  • Record ID : 1985-2686
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 2
  • Publication date: 1985

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