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THE INFLUENCE OF COOLING RATE AND WARMING RATE ON THE RESPONSE OF RENAL CORTICAL SLICES FROZEN TO 233 K (-40 DEG C) IN THE PRESENCE OF 2.1 M CRYOPROTECTANT (ETHYLENE GLYCOL, GLYCEROL, OR DMSO).

Author(s) : HAWKINS H. E., CLARK P., KAROW A. M. Jr

Type of article: Article

Summary

RENAL CORTICAL SLICES WERE TREATED WITH 2.1 M CRYOPROTECTANT IN RPS-2 VEHICLE SOLUTION, COOLED AT ONE OF FOUR RATES TO 233 K, THEN IMMEDIATELY WARMED AT ONE OF FOUR RATES TO 298 K (25 DEG C) FOR DETERMINATION OF THE RATE OF POTASSIUM-SODIUM IONS AFTER A STANDARD INCUBATION PERIOD. THE RESULTS SUGGEST THAT DMSO OFFERS THE PROSPECT FOR GREATEST POST-THAW RECOVERY.

Details

  • Original title: THE INFLUENCE OF COOLING RATE AND WARMING RATE ON THE RESPONSE OF RENAL CORTICAL SLICES FROZEN TO 233 K (-40 DEG C) IN THE PRESENCE OF 2.1 M CRYOPROTECTANT (ETHYLENE GLYCOL, GLYCEROL, OR DMSO).
  • Record ID : 1986-1715
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 4
  • Publication date: 1985

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