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The rehydration injury study of freeze-dried red blood cells during rehydration.

Author(s) : SHU Z. Q., LIU Z., CHEN W. W., et al.

Summary

The injuries caused during rehydration, including membranes phase transition and volume response, are very important factors that lead to low recovery rate of freeze-dried red blood cells after rehydration. During rehydration, the membranes of lyophilized red blood cells maybe suffer phase transition because membranes with different water contents have different phase transition temperatures. At the same time, the osmolality of the environmental solution and the volumes of the cells change greatly. If the rehydration temperature and procedures are not optimum, the injuries caused by the leakage of intracellular substances and extreme volume response of the cells will occur. In order to explore the phase transition of the membranes of lyophilized red blood cells, the ghosts of red blood cells were prepared and freeze dried. Their phase transition temperatures were measured to be about 10 and 32-34°C, with differential scanning calorimeter. The membrane's phase transitions of freeze dried red blood cells before, during and after rehydration were analyzed. In order to avoid the injury caused by the phase transitions of the membranes, the rehydration temperature should be above 34°C. In experiments, the red blood cells were freeze-dried and rehydrated at different temperatures. This work implies the injury mechanism of rehydration of freeze-dried red blood cells caused by phase transitions and cell volume response. It's also helpful for the freeze-drying preservation of other cells.

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Pages: ICR07-C1-1499

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Details

  • Original title: The rehydration injury study of freeze-dried red blood cells during rehydration.
  • Record ID : 2008-0955
  • Languages: English
  • Source: ICR 2007. Refrigeration Creates the Future. Proceedings of the 22nd IIR International Congress of Refrigeration.
  • Publication date: 2007/08/21

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