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THE STRUCTURAL STABILITY OF ESCHERICHIA COLI CELL MEMBRANES RELATED TO THE RESISTANCE OF THE CELLS TO FREEZE-THAWING AND FREEZE-DRYING.

Author(s) : SOUZU H.

Summary

CYTOPLASMIC AND OUTER MEMBRANE FROM ESCHERICHIA COLI IN DIFFERENT GROWTH PHASES HAVE DIFFERENT CHEMICAL COMPOSITIONS. IN FREEZING, LOGARITHMIC PHASE CELLS SHOWED A MARKED PERMEABILITY INCREASE IN THE MEMBRANE AS WELL AS A RELEASE OF CELL MEMBRANE COMPONENTS, WHEREAS FREEZING IN THE STATIONARY PHASE CELLS DIDNOT SHOW ANY CHANGE IN PERMEABILITY AND CHEMICAL NATURE. IN FREEZE-THAWING AND FREEZE-DRYING, LESSER CELL SURVIVALS IN LOGARITHMIC PHASE CELLS THAN IN THE STATIONARY PHASE CELLS WERE SEEN. COOLING OF MEMBRANE LIPIDS WITH TRANS-PARINARIC ACID SHOWED A DISTINCT INCREASE OF FLUORESCENCE POLARIZATION FROM ROOM TEMPERATURE TO BELOW 273 K (0 DEG C). THE MEMBRANE ALSO EXHIBITED A TEMPERATURE-DEPENDENT FLUORESCENCE POLARIZATION CHANGE, HOWEVER, THE LESSER RATIO OF POLARIZATION WAS OBSERVED IN THE CELLS IN ADVANCED GROWTH PHASES.

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Details

  • Original title: THE STRUCTURAL STABILITY OF ESCHERICHIA COLI CELL MEMBRANES RELATED TO THE RESISTANCE OF THE CELLS TO FREEZE-THAWING AND FREEZE-DRYING.
  • Record ID : 1987-1206
  • Languages: English
  • Source: Fundamentals and Applications of Freeze-Drying to Biological Materials, Drugs and Foodstuffs.
  • Publication date: 1985/05/20
  • Document available for consultation in the library of the IIR headquarters only.

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