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The ultrastructure of the embryonic avian eye after high pressure freezing and freeze substitution.

Author(s) : CACCHILLO P. F., ALLENSPACH A. L.

Type of article: Article

Summary

Chick embryo eyes (stages 27-31) were cryofixed by high pressure and freeze substitution for comparison of morphology with that of conventionally fixed eyes. Quality cryopreservation was accomplished to depths of 80-100 micrometers. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) reveal smooth cellular surfaces and lack of intracellular vacuoles. Corneal stroma is cryofixed as a thick, compact layer of amorphous matrix which embeds fibrillar collagen. This technique demonstrates improved preservation of external cellular morphology and preservation of matrix by freeze substitution solutions.

Details

  • Original title: The ultrastructure of the embryonic avian eye after high pressure freezing and freeze substitution.
  • Record ID : 1993-0536
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 3
  • Publication date: 1992/05
  • Document available for consultation in the library of the IIR headquarters only.

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