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Cryoprotective effect of egg yolk in cryopreservation of porcine embryos.

Author(s) : FUJINO Y., UJISATO Y., ENDO K., TOMIZUKA T., KOJIMA T., OGURI N.

Type of article: Article

Summary

The objective of the present study was to assess the effect of egg yolk supplementation in solutions for either cooling or freezing of porcine embryos. In experiment 1, porcine embryos collected on day 3 to 7 (day 0 = onset of estrus) were allocated to 12 experimental groups according to their developmental stages and the concentrations of egg yolk for cooling to 0 deg C. Whereas no embryos ranging from four-cell to blastocysts survived, the 10-minute exposure to 0 deg C despite egg yolk supplementation, expanded blastocysts and hatched blastocysts allocated to egg yolk-supplemented groups survived cooling to 0 deg C. In experiment 2, porcine expanded and hatched blastocysts collected on day 6 or 7 were assigned to six experimental groups according to their developmental stages and the type of cryoprotectant used for freezing at -196 deg C.

Details

  • Original title: Cryoprotective effect of egg yolk in cryopreservation of porcine embryos.
  • Record ID : 1994-1870
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 3
  • Publication date: 1993/06

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