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VISUALIZATION OF FREEZING DAMAGE TO BIOLOGICAL CELLS: A VIDEODEMONSTRATION OF CRYOMICROSCOPY.

Author(s) : ENGLICH S., KORBER C., RAU G.

Summary

CRYOMICROSCOPY PROVIDES A DIRECT ANALYSIS OF THE EFFECT OF FREEZE/THAW ON CELLS AND THEIR VIABILITY (BLOOD CELLS, MAINLY LYMPHOCYTES). AFTER MAZUR, THE PRESENCE OF SOLUTIONS AT HIGH CONCENTRATION AT SUBOPTIMAL COOLING RATES AND THE FORMATION OF INTRACELLULAR ICE AT HIGH COOLING RATES ARE THE FACTORS RESPONSIBLE FOR THE LOW SURVIVAL RATE OF CELLS. DENSITOMETRIC STUDIES SHOW CONCENTRATION PROFILES BEFORE THE ICE FRONT. INTRACELLULAR ICE CRYSTALS ARE FORMED ABOVE THE OPTIMUM COOLING RATE ; THERE ARE TWO CATEGORIES OF ICE CRYSTALS: < DARKENING > OF CELL DUE TO LIGHT DISPERSION AND < TWITCHING >, SUDDEN MOVEMENT TO BE OBSERVED ONLY DYNAMICALLY. PENETRATING CRYOPROTECTANTS SUCH AS DMSO AND NON-PENETRATING ONES SUCH AS HES CHANGE THE CRYSTALLIZATION TEMPERATURES AND STRUCTURES.

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Pages: 131-134

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Details

  • Original title: VISUALIZATION OF FREEZING DAMAGE TO BIOLOGICAL CELLS: A VIDEODEMONSTRATION OF CRYOMICROSCOPY.
  • Record ID : 1988-1679
  • Languages: English
  • Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
  • Publication date: 1987/08/24
  • Document available for consultation in the library of the IIR headquarters only.

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