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A FLUOROMETRIC ASSAY OF CELL MEMBRANE INTEGRITY OF FROZEN LYMPHOCYTES.

Author(s) : ZHENG S.

Summary

LYMPHOCYTES OF HUMAN PERIPHERAL BLOOD WERE FROZEN AT 253, 233 AND 77 K (-20, -40 AND -196 DEG C) FOR 3 MIN. A COMBINATION OF 2 FLUORESCENT DYES: FLUORESCEIN DIACETATE (FDA) AND ETHIDIUM BROMIDE, WAS USED TO TEST CELL VIABILITY. LYMPHOCYTE MEMBRANES ARE SUBJECT TO A DIFFERENT DEGREE OF DAMAGE WHEN THE FREEZING MEDIUM IS FREE FROM CRYOPROTECTANT. THERE WAS SIGNIFICANT DECREASE IN THE NUMBER OF LABELLED CELLS AND THE ACCUMULATED AMOUNT OF FDA, ESPECIALLY WHEN THE LYMPHOCYTES WERE FROZEN AT 233 K. WHEN LYMPHOCYTES WERE FROZEN WITH 10% DMSO, A HIGH FLUORESCENT REACTION WAS OBSERVED. DMSO DECREASED THE INTENSITY OF FDA ACCUMULATED IN LYMPHOCYTES AT TEMPERATURES ABOVE 273 K (0 DEG C).

Details

  • Original title: A FLUOROMETRIC ASSAY OF CELL MEMBRANE INTEGRITY OF FROZEN LYMPHOCYTES.
  • Record ID : 1991-0880
  • Languages: English
  • Publication date: 1989/10/11
  • Source: Source: Proc. 7th int. Congr. Cryosurg., Beijing
    234-238; 4 fig.; 1 tabl.; 5 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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