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Vitrification of mature mouse oocytes in a 6 M dimethyl sulfoxide solution supplemented with antifreeze glycoproteins: the effect of temperature.

Author(s) : O'NEIL L., PAYNTER S. J., FULLER B. J., SHAW R. W., DEVRIES A. L.

Type of article: Article

Summary

Of the oocytes cryopreserved following exposure to vitrification in 6 mole dimethyl sulfoxide supplemented with 1mg/ml antifreeze glycoproteins (solution known as VSD + AFGP) at room temperature, the overall percentage development to blastocyst was 20%. Exposure of oocytes to the VSD + AFGP on ice prior to cryopreservation yielded significantly improved rates of fertilization (94%) and overall development to blastocyst (66%) when compared with oocytes cryopreserved following exposure to the VSD + AFGP at room temperature. Rates of normality and development to blastocyst were also improved. Cryopreservation in 6 M dimethyl sulfoxide supplemented with 1 mg/ml AFGP resulted in poor rates of survival which were highly variable when exposure to cryoprotective agent (CPA) was performed at room temperature. Lowering the temperature of exposure to CPA prior to cryopreservation resulted in improved viability.

Details

  • Original title: Vitrification of mature mouse oocytes in a 6 M dimethyl sulfoxide solution supplemented with antifreeze glycoproteins: the effect of temperature.
  • Record ID : 1999-3905
  • Languages: English
  • Source: Cryobiology - vol. 37 - n. 1
  • Publication date: 1998/08

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