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A CRYOMICROSCOPE USING DIRECTIONAL SOLIDIFICATION FOR THE CONTROLLED FREEZING OF BIOLOGICAL MATERIAL.

Author(s) : RUBINSKY B., IKEDA M.

Type of article: Article

Summary

A NEW CRYOMICROSCOPE STAGE WAS DESIGNED IN WHICH LARGE SAMPLES OF BIOLOGICAL MATERIALS CAN BE FROZEN IN A PREDETERMINED DIRECTION WITH A CONSTANT CONTROLLED COOLING RATE. THE CONCEPT AS WELL AS THE DETAILS OF THE CRYOMICROSCOPE ARE PRESENTED. DIRECTIONAL SOLIDIFICATION OF PHYSIOLOGICAL SALINE AND SALINE SUSPENSIONS OF ERYTHROCYTES AT CONSTANT RATES VERIFIED THE SUCCESSFUL OPERATION OF THE CRYOMICROSCOPE AT CONSTANT COOLING RATES. RESULTS SHOW THAT THE FREEZING PATTERN IN PHYSIOLOGICAL SALINE AND SALINE SUSPENSIONS OF ERYTHROCYTES ARE IDENTICAL.

Details

  • Original title: A CRYOMICROSCOPE USING DIRECTIONAL SOLIDIFICATION FOR THE CONTROLLED FREEZING OF BIOLOGICAL MATERIAL.
  • Record ID : 1985-2692
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 1
  • Publication date: 1985

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