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A metabolic view of receptor activation in cultured cells following cryopreservation.

Author(s) : KUO R. C., BAXTER G. T., ALAJOKI L., MILLER D.L., LIBBY J. M., OWICKI J. C.

Type of article: Article

Summary

The effect of cryopreservation on agonist-induced receptor activation in mammalian cells was investigated with the cytosensor microphysiometer, a biosensor that monitors cellular metabolic activity by measuring changes in extracellular pH. Two different cell types were cryopreserved by freezing in a disposable cell capsule used in the microphysiometer. Cryopreservation caused no detectable difference in desensitization of the response due to repeated application of carbachol. These results demonstrate the feasibility of pharmacological studies with cryopreserved cells in the microphysiometer and further suggest that the microphysiometer may be useful in exploring the biological consequences of cryopreservation in the early post-thaw period.

Details

  • Original title: A metabolic view of receptor activation in cultured cells following cryopreservation.
  • Record ID : 1994-1867
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 4
  • Publication date: 1993/08

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