Postthaw viability of precultured hepatocytes.

Author(s) : DARR T. B., HUBEL A.

Type of article: Article


Potential applications of hepatocytes include drug metabolism studies, gene therapy and use in liver-assist devices. Cryopreservation of hepatocytes would enable subsequent pooling of cells under safety- and quality-controlled conditions. In this study, various cryopreservation techniques and cryoprotectants were investigated. Results indicate that use of preliminary culture before cryopreservation can enhance hepatocyte function following thawing.


  • Original title: Postthaw viability of precultured hepatocytes.
  • Record ID : 2002-1631
  • Languages: English
  • Source: Cryobiology - vol. 42 - n. 1
  • Publication date: 2001/02


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