Postthaw viability of precultured hepatocytes.
Author(s) : DARR T. B., HUBEL A.
Type of article: Article
Summary
Potential applications of hepatocytes include drug metabolism studies, gene therapy and use in liver-assist devices. Cryopreservation of hepatocytes would enable subsequent pooling of cells under safety- and quality-controlled conditions. In this study, various cryopreservation techniques and cryoprotectants were investigated. Results indicate that use of preliminary culture before cryopreservation can enhance hepatocyte function following thawing.
Details
- Original title: Postthaw viability of precultured hepatocytes.
- Record ID : 2002-1631
- Languages: English
- Source: Cryobiology - vol. 42 - n. 1
- Publication date: 2001/02
Links
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Thawing; Cryobiology; Cell; Survival; Cryopreservation; Liver
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