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A new method for studying unfrozen water content of biological tissues during freezing.

Author(s) : XU Y., HUA T., ZHOU G., et al.

Summary

Unfrozen water content is essential for analyses of cell livability during cryopreservation protocols as well as cryosurgery. This study shows a new method for studying the unfrozen water content of biological tissues during freezing. Stepwise scanning-DSC (SW-DSC) is firstly introduced to study the unfrozen water content of rabbit aorta during freezing. This study provides insight into the effect of presence of cryoprotectants (CPA) on unfrozen water content further. Results show that the unfrozen water content of rabbit aorta can be precisely determined by the technique of SW-DSC within a cryogenic temperature range(such as 0 to -50°C). And, unfrozen water content of fresh aorta (i.e. without CPA) decreases rapidly as temperature lowing (e.g. unfrozen water content changes from 1 to 0.32). However, fresh sample permeated by 10% (v/v) dimethylsulfoxide (DMSO, a kind of CPA) solution, unfrozen water content of the sample decreases slowly(e.g. from 1 to 0.45), which means that the unfrozen water content increases significantly with DMSO concentration increasing at the same temperature due to the strong hydration action of DMSO.

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Pages: ICR07-C1-802

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Details

  • Original title: A new method for studying unfrozen water content of biological tissues during freezing.
  • Record ID : 2007-2825
  • Languages: English
  • Source: ICR 2007. Refrigeration Creates the Future. Proceedings of the 22nd IIR International Congress of Refrigeration.
  • Publication date: 2007/08/21

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