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A numerical study of cell behaviour in a ternary solution during the freezing process.

Author(s) : LUO D., HE L., CHENG S., et al.

Type of article: Article

Summary

Using a continuum model for multi-component phase change system, the freezing of cell suspension in a ternary solution, H2O-NaCl-CPA (cryoprotective agent) inside a flat bag was investigated numerically in this study. The temperature and phase change history, intracellular water loss, and the volume change of the cells at different locations inside cell suspension are calculated. Numerical results revealed that although the sample boundary was cooled at a constant rate, different locations inside the sample experienced different temperature changes and cooling rates. The highest cooling rates occurerd at internal locations. The cell volume change was location-dependent.

Details

  • Original title: A numerical study of cell behaviour in a ternary solution during the freezing process.
  • Record ID : 2004-0410
  • Languages: English
  • Source: CryoLetters - vol. 24 - n. 3
  • Publication date: 2003/05
  • Document available for consultation in the library of the IIR headquarters only.

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