A SIMPLE TECHNIQUE FOR CRYOPRESERVATION OF THE RUMEN PROTOZOA ENTODINIUM SIMPLEX.
Author(s) : MARCIN A.
Type of article: Article
Summary
THE FIRST SUCCESSFUL CRYOPRESERVATION OF THE RUMEN PROTOZOA ENTODINIUM SIMPLEX WAS CARRIED OUT USING A SIMPLE TECHNIQUE IN THE COOLING VESSEL. THE COOLING VESSEL WAS PLACED ABOVE THE LEVEL OF LIQUIDNITROGEN. THE OPTIMAL SURVIVAL 15% WAS ACHIEVED USING 0.38 MILLILITRE/LITRE OF DMSO AS A CRYOPROTECTANT FOR 30 MIN EQUILIBRATION AT THE CONCENTRATION OF 80,000 PROTOZOA PER MILLILITRE AND AVERAGE COOLING RATE FROM 0.5 TO 2.2 K PER MIN.
Details
- Original title: A SIMPLE TECHNIQUE FOR CRYOPRESERVATION OF THE RUMEN PROTOZOA ENTODINIUM SIMPLEX.
- Record ID : 1989-2517
- Languages: English
- Source: Cryo-Letters - vol. 10 - n. 2
- Publication date: 1989/03
- Document available for consultation in the library of the IIR headquarters only.
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Indexing
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Long-term cryopreservation of Toxoplasma gondii.
- Author(s) : POPESCU C., STERIU D.
- Date : 1993/11
- Languages : English
- Source: Cryo-Letters - vol. 14 - n. 6
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CRYOPRESERVATION OF GIARDIA LAMBLIA WITH DIMETH...
- Author(s) : LYMAN J. R., MARCHIN G. L.
- Date : 1984
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- Source: Cryobiology - vol. 21 - n. 2
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- Author(s) : SANDSKAR B., MAGALHAES B.
- Date : 1994/04
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- Source: Cryobiology - vol. 31 - n. 2
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Cryopreservation of living organs.
- Author(s) : TANASAWA I., NAGATA S., KIMURA N.
- Date : 1993
- Languages : Japanese
- Source: Trans. JAR - vol. 10 - n. 1
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A simple technique for cryopreservation of the ...
- Author(s) : MARCIN A., et al.
- Date : 1992/05
- Languages : English
- Source: Cryo-Letters - vol. 13 - n. 3
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