ShareA simple technique for cryopreservation of the rumen protozoan Entodinium caudatum.
Author(s) : MARCIN A., et al.
Type of article: Article
Summary
The first successful method for the cryopreservation of the rumen protozoan Entodinium caudatum is described. It was carried out using a simple technique. The cooling vessel with samples was placed in liquid nitrogen vapour during cooling from 312.2 to 173.15 K. The cryopreserved samples were immediately immersed into liquid nitrogen at 173.15 K. Optimal survival of 7.55% was achieved using 0.38 mol/l of dimethylsulfoxide as a cryoprotectant for 20 min equilibration at the average concentration of 30,000-40,000 protozoan cells per ml and average cooling rate 2-3 K/min.
Details
- Original title: A simple technique for cryopreservation of the rumen protozoan Entodinium caudatum.
- Record ID : 1993-0517
- Languages: English
- Source: Cryo-Letters - vol. 13 - n. 3
- Publication date: 1992/05
- Document available for consultation in the library of the IIR headquarters only.
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A SIMPLE TECHNIQUE FOR CRYOPRESERVATION OF THE ...
- Author(s) : MARCIN A.
- Date : 1989/03
- Languages : English
- Source: Cryo-Letters - vol. 10 - n. 2
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Cryopreservation of living organs.
- Author(s) : TANASAWA I., NAGATA S., KIMURA N.
- Date : 1993
- Languages : Japanese
- Source: Trans. JAR - vol. 10 - n. 1
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The effect of growth phase, cryoprotectants and...
- Author(s) : PÉTER G., REICHART O.
- Date : 2001/03
- Languages : English
- Source: Acta Aliment. - vol. 30 - n. 1
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FREEZING AND THAWING INDUCED CURING OF DRUG-RES...
- Author(s) : CALCOTT P., WOOD D., ANDERSON L.
- Date : 1983
- Languages : English
- Source: Cryo-Letters - vol. 4 - n. 2
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Long-term cryopreservation of Toxoplasma gondii.
- Author(s) : POPESCU C., STERIU D.
- Date : 1993/11
- Languages : English
- Source: Cryo-Letters - vol. 14 - n. 6
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