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ACROSOMAL DAMAGE AND ENZYME LEAKAGE DURING FREEZE PRESERVATION OF BUFFALO SPERMATOZOA.

Author(s) : KAKAR S. S., ANAND S. R.

Type of article: Article

Summary

SEMEN OF MURRAH BUFFALO BULLS WAS FROZEN IN 4 DILUENTS, VIZ EGG YOLK-CITRATE-GLUCOSE, EGG YOLK-TRIS, SKIM MILK-EGG YOLK AND CITRIC ACID WHEY. IN FRESHLY EJACULATED SEMEN, ABOUT 80% OF SPERMATOZOA WERE SCORED TO HAVE NORMAL HEAD MORPHOLOGY. MEASUREMENT OF BUFFALO SPERM STRUCTURE USING GIEMSA STAIN REVEALED SIGNIFICANT (P LOWER THAN 0.01) DAMAGE TO ACROSOME ON FREEZING WHICH VARIED BETWEEN 37-42% IN 4 DILUENTS. EXAMINATION WITH SCANNING ELECTRON MICROSCOPE SHOWED GREATER DAMAGE AFTER FREEZING AND THAWING WITH 13-22% OF SPERM HEADS HAVING NORMAL ACROSOME STRUCTURE IN 4 DILUENTS.

Details

  • Original title: ACROSOMAL DAMAGE AND ENZYME LEAKAGE DURING FREEZE PRESERVATION OF BUFFALO SPERMATOZOA.
  • Record ID : 1985-0348
  • Languages: English
  • Source: Indian J. exp. Biol. - vol. 22 - n. 1
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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