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A COMPARATIVE STUDY IN METHODS OF CRYOPROTECTION FOR HUMAN SEMEN.

Author(s) : HARRISON R. F., SHEPPARD B. L.

Type of article: Article

Summary

A COMPARATIVE STUDY USING EITHER GLYCEROL OR AN EGG YOLK-CITRATE-GLYCEROL MIX FOR CRYOPROTECTION SHOWED THE LATTER TO GIVE A SIGNIFICANTLY BETTER POST-THAW MOTILITY. THE GREATEST DROP WAS NOTED WITHIN THE FIRST HOUR, SUGGESTING THAT FREEZABILITY OF A SAMPLE COULD BE JUDGED ACCURATELY BY RETHAWING AT THAT TIME. CONTROLLED STUDIES USING SCANNING ELECTRON MICROSCOPY CLEARLY SHOWED A GREATER HEAD DISRUPTION AFTER FREEZING WITH GLYCEROL AND LOOPED TAILS AFTER CENTRIFUGATION. THESE FINDINGS ARE REVIEWED AND IT IS RECOMMENDED THAT SEMINAL SAMPLES ARE NOT CENTRIFUGED AND THAT A COMPLEX MEDIUM BE EMPLOYED FOR SEMINAL FREEZING AND STORING.

Details

  • Original title: A COMPARATIVE STUDY IN METHODS OF CRYOPROTECTION FOR HUMAN SEMEN.
  • Record ID : 1981-0319
  • Languages: English
  • Source: Cryobiology - vol. 17 - n. 1
  • Publication date: 1980/02

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