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AN ASSESSMENT OF TUMOUR CELL VIABILITY AFTER IN VITRO FREEZING.

Author(s) : JACOB G., KURZER M. N., FULLER B. J.

Type of article: Article

Summary

THE IDENTIFICATION OF THE MINIMUM LETHAL TEMPERATURE FOR TUMOUR CELLS IN VIVO IS DIFFICULT BECAUSE OF THE SECONDARY FACTORS THAT ARE ASSOCIATED WITH THE CRYOINJURY. THIS STUDY ATTEMPTS TO IDENTIFY THIS TEMPERATURE BY A COMBINATION OF IN VITRO AND IN VIVO TECHNIQUES. LESS THAN 1% OF THE CELLS FROZEN TO 238 K (-35 DEG C) WERE CONSIDERED VIABLE, YET SIGNIFICANT TUMOUR TAKE RATES WERE NOTED, SUGGESTING THAT FOR SOME CELLS THE CRYOINJURY IS REVERSIBLE. AS EXPECTED TUMOUR TAKE RATES WERE REDUCED BY LOWERING THE TEMPERATURE BUT WERE INDEPENDENT OF THE HOLDING TIME. THE MINIMUM LETHAL TEMPERATURE WAS DEPENDENT UPON THE SITE OF INOCULATION, SUBCUTANEOUS TUMOURS DEVELOPING FROM CELLS FROZEN TO 238 K, WHEREAS LIVER TUMOURS DID NOT DEVELOP FROM CELLS FROZEN BEYOND 248 K (-25 DEG C), THIS MAY HAVE IMPORTANT CLINICAL IMPLICATIONS.

Details

  • Original title: AN ASSESSMENT OF TUMOUR CELL VIABILITY AFTER IN VITRO FREEZING.
  • Record ID : 1986-2115
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 5
  • Publication date: 1985

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