An evaluation of the preservability of modified UW solutions using the isolated rat hepatocytes.
Author(s) : KAGAYA H., ISHII F., HANADA K., ISHIBASHI Y., ARAI T., SATO K., KAKITA A., SHIMADA S., OGATA H.
Type of article: Article
Summary
The study demonstrated that glutathione and adenosine were essential for the successful preservation, whereas hydroxyethyl starch (HES) and allopurinol could be omitted from University of Wisconsin (UW) solution without remarkable reduction of preservability. Raffinose and lactobonate might be required simply for maintaining the osmotic pressure of the solutions, because they were replaceable with sodium chloride. It was also found that HES could be replaced by Dextran 40 without significant cell deterioration. This technique seems to permit rapid assessment and refinement of new solutions for liver preservation prior to testing in a large animal transplant model.
Details
- Original title: An evaluation of the preservability of modified UW solutions using the isolated rat hepatocytes.
- Record ID : 1998-1295
- Languages: English
- Source: Cryobiology - vol. 22 - n. 4
- Publication date: 1996/12
Links
See other articles in this issue (6)
See the source
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cell; Rat; Survival; Cryopreservation; Liver
-
Evaluation of preservability of isolated rat he...
- Author(s) : KAGAYA H., HANADA K., ISHIBASHI Y., ARAI T., MASAKI Y., SHIMADA S., OGATA H.
- Date : 1996/06
- Languages : English
- Source: Low Temperature Medicine - vol. 22 - n. 2
View record
-
Survival, metabolic activity and transport of p...
- Author(s) : PETRENKO A. Ju., GRISCHUK B. P., ROSLYAKOV A. D., et al.
- Date : 1992/03
- Languages : English
- Source: Cryo-Letters - vol. 13 - n. 2
View record
-
A method for the cryopreservation of liver pare...
- Author(s) : DIENER B., UTESCH D., BEER N., DÜRK H., OESCH F.
- Date : 1993/04
- Languages : English
- Source: Cryobiology - vol. 30 - n. 2
View record
-
STUDIES ON CRYOPROTECTANT EQUILIBRATION IN THE ...
- Author(s) : FULLER B. J., BUSZA A. L., PROCTOR E.
- Date : 1989
- Languages : English
- Source: Cryobiology - vol. 26 - n. 2
View record
-
CRYOPRESERVATION OF ISOLATED RAT HEPATOCYTES: A...
- Author(s) : CHESNE C., GUILLOUZO A.
- Date : 1988
- Languages : English
- Source: Cryobiology - vol. 25 - n. 4
View record