Share

Survival, metabolic activity and transport of potassium ions of rat hepatocytes after rapid freeze-thawing under protection of dimethylsulfoxide and separation in percoll density gradient.

Author(s) : PETRENKO A. Ju., GRISCHUK B. P., ROSLYAKOV A. D., et al.

Type of article: Article

Summary

Viability of rat hepatocytes were studied after different conditions of cryopreservation under protection of dimethylsulfoxide, involving rapid two-stage freezing. Cell survival to the least extent decreased after slow administration of cryoprotectant on ice, rapid freezing of suspension with the density of 5-15 millions cells/ml down to -196 deg C with a stoppage at -20/-25 deg C and rapid thawing. A comparative investigation has been conducted. Conditions of this investigation are described as well as results. The results obtained testify to the fact that hepatocytes, which have maintained the integrity of plasma membrane, are functionally active.

Details

  • Original title: Survival, metabolic activity and transport of potassium ions of rat hepatocytes after rapid freeze-thawing under protection of dimethylsulfoxide and separation in percoll density gradient.
  • Record ID : 1993-0546
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 2
  • Publication date: 1992/03
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (5)
See the source

Indexing