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CRYOPRESERVATION OF ISOLATED RAT HEPATOCYTES: A CRITICAL EVALUATION OF FREEZING AND THAWING CONDITIONS.

Author(s) : CHESNE C., GUILLOUZO A.

Type of article: Article

Summary

VARIOUS PARAMETERS, INCLUDING THE NATURE AND PROPORTION OF THE CONSTITUENTS OF THE CRYOPROTECTIVE MEDIUM, THE COOLING RATE, AND THE COMPOSITION OF THE THAWING MEDIUM, WERE EVALUATED. THE HIGHEST PERCENTAGE OF CELLS ABLE TO SURVIVE IN CULTURE WAS OBTAINED BY FREEZING IN L15 MEDIUM CONTAINING 16% DMSO, AT 3 K/MIN, AND BY ADDING 0.8 M GLUCOSE TO THE THAWING MEDIUM. MORE THAN 50% OF HEPATOCYTES CAPABLE OFATTACHMENT JUST AFTER CELL ISOLATION KEPT THIS PROPERTY AFTER FREEZING AND SURVIVED IN PRIMARY CULTURE.

Details

  • Original title: CRYOPRESERVATION OF ISOLATED RAT HEPATOCYTES: A CRITICAL EVALUATION OF FREEZING AND THAWING CONDITIONS.
  • Record ID : 1989-2077
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 4
  • Publication date: 1988

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