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EFFECT OF COOLING RATE ON THE CRYOPRESERVATION OF NEONATAL SPRAGUE-DAWLEY PRIMARY CULTURED HEPATOCYTES.

Author(s) : BEECHERL E. E.

Type of article: Article

Summary

PRIMARY CULTURES OF NEONATAL RAT HEPATOCYTES WERE USED TO ESTABLISH AN OPTIMAL COOLING RATE FOR CRYOPRESERVATION. CELLS WERE SUSPENDED IN A SOLUTION OF 2 M DMSO, NUCLEATED AT 263 K (-10 DEG C), AND COOLED TO 213 K (-60 DEG C) AT ONE OF SIX CONSTANT COOLING RATES FROM 0.5 TO 20 K PER MIN. AFTER THAWING AT A WARMING RATE OF 100 K PER MIN AND DILUTION OF DMSO, CELL VIABILITY WAS DETERMINED BY DYE EXCLUSION. OPTIMAL SURVIVAL WAS OBSERVED AT A COOLING RATE OF 10 K PER MIN.

Details

  • Original title: EFFECT OF COOLING RATE ON THE CRYOPRESERVATION OF NEONATAL SPRAGUE-DAWLEY PRIMARY CULTURED HEPATOCYTES.
  • Record ID : 1988-0827
  • Languages: English
  • Source: Cryo-Letters - vol. 8 - n. 5
  • Publication date: 1987
  • Document available for consultation in the library of the IIR headquarters only.

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