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CHLOROPLAST MEMBRANE DAMAGE DURING FREEZING: THE LIPID PHASE.

Author(s) : JENSEN M., HEBER U., OETTMEIER W.

Type of article: Article

Summary

IN ORDER TO STUDY THE EFFECT OF FREEZE DAMAGE TO CHLOROPLAST MEMBRANES MICROVISCOSITY OF SPINACH THYLAKOIDS WAS PROBED BY STEARIC ACID SPIN LABELS. IN CRYOPROTECTED CHLOROPLAST MEMBRANES ARRHENIUS PLOT BREAKS INDICATIVE OF PHASE CHANGES ARE OBSERVED AT 288 AND 263 K (15 AND -10 DEG C). BREAKS IN THE ARRHENIUS PLOTS WERE NOT OBSERVED ON VESICLES PREPARED FROM CHLOROPLAST LIPIDS BY SONICATION. INSTEAD, A MELTING ZONE WAS INDICATED BELOW 243K (-30 DEG C). IT IS SUGGESTED THAT FREEZING WHICH IS KNOWN TO DISSOCIATE PROTEINS FROM THE MEMBRANES ALTERED THE CHARGE DISTRIBUTION OF THE MEMBRANES LEADING FIRST TO MEMBRANE SWELLING AND FINALLY, BY THE OPENING OF HYDROPHILIC CHANNELS, TO MEMBRANE COLLAPSE.

Details

  • Original title: CHLOROPLAST MEMBRANE DAMAGE DURING FREEZING: THE LIPID PHASE.
  • Record ID : 1982-0832
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 3
  • Publication date: 1981/06

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