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Choosing a cryoprotectant for maintenance of biotransforming activity of hepatocytes, cryopreserved by two-stage rapid freeze-thawing.

Author(s) : MAZUR S. P., PETRENKO A. Ju., ROSLYAKOV A. D.

Type of article: Article

Summary

Suspensions of rat hepatocytes were frozen and thawed. Different cryoprotectants are used (DMSO, glycerol, dimethylacetamide, 1.2-propanediol). DMSO and glycerol maintain 62-64% of their original biotransforming activity. Glycerol possesses the advantage of avoiding a stage of cryoprotectant washing.

Details

  • Original title: Choosing a cryoprotectant for maintenance of biotransforming activity of hepatocytes, cryopreserved by two-stage rapid freeze-thawing.
  • Record ID : 1994-1882
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 1
  • Publication date: 1993/01
  • Document available for consultation in the library of the IIR headquarters only.

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