ShareCRYOPRESERVATION OF ISOLATED RAT HEPATOCYTES IN PELLET FORM.
Author(s) : FULLER B. J., JAMES E.
Type of article: Article
Summary
CELL SUSPENSIONS WERE COOLED SLOWLY AS 50 MICROL DROPLETS USING DMSO AS PROTECTANT. THE FROZEN DROPLETS WERE FOUND TO BE A CONVENIENT METHOD FOR STORING LARGE NUMBERS OF HEPATOCYTES WHILST ALLOWING GREAT FLEXIBILITY IN THE NUMBER RECOVERED AT ANY ONE TIME FOR EXPERIMENTATION. METABOLIC ACTIVITY OF CELLS STORED AT 77 K (-196 DEG C) AS PELLETS WAS SIMILAR TO THAT SEEN IN HEPATOCYTES CRYOPRESERVED IN CONVENTIONAL GLASS AMPOULES.
Details
- Original title: CRYOPRESERVATION OF ISOLATED RAT HEPATOCYTES IN PELLET FORM.
- Record ID : 1985-2228
- Languages: English
- Source: Cryo-Letters - vol. 6 - n. 1
- Publication date: 1985
- Document available for consultation in the library of the IIR headquarters only.
Links
See other articles in this issue (1)
See the source
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryoprotectant; Cell; DMSO; Rat; Cryopreservation; Liver
-
THE BIOENERGETICS OF MITOCHONDRIA AFTER CRYOPRE...
- Author(s) : FULLER B. J.
- Date : 1989
- Languages : English
- Source: Cryobiology - vol. 26 - n. 4
View record
-
THE EFFECTS OF CRYOPRESERVATION ON MEMBRANE INT...
- Author(s) : LOECKER R. de
- Date : 1990
- Languages : English
- Source: Cryobiology - vol. 27 - n. 2
View record
-
STUDIES ON CRYOPROTECTANT EQUILIBRATION IN THE ...
- Author(s) : FULLER B. J., BUSZA A. L., PROCTOR E.
- Date : 1989
- Languages : English
- Source: Cryobiology - vol. 26 - n. 2
View record
-
A NEWLY DEVELOPED SYSTEM FOR COOLING AND PRESER...
- Author(s) : KUSUMOTO K.
- Date : 1987
- Languages : Japanese
- Source: Low Temperature Medicine - vol. 13 - n. 2
View record
-
CRYOPRESERVATION OF ISOLATED RAT HEPATOCYTES: A...
- Author(s) : CHESNE C., GUILLOUZO A.
- Date : 1988
- Languages : English
- Source: Cryobiology - vol. 25 - n. 4
View record