COLD SHOCK AND ITS PREVENTION BY EGG YOLK IN SPERMATOZOA OF THE CAT.
Author(s) : GLOVER T. E., WATSON P. F.
Type of article: Article
Summary
CAT SPERMATOZOA WERE SUBJECTED TO REPEATED RAPID COOLING TO 273 K (0 DEG C) EITHER UNDILUTED OR EXTENDED IN AN EGG YOLK DILUENT. A SIGNIFICANT DECLINE IN MOTILITY OCCURRED WITH EACH SUCCESSIVE COOLING CYCLE, BUT EGG YOLK REDUCED THE MAGNITUDE OF THE EFFECT. SLOW COOLING OVERPERIODS FROM 11 TO 300 MIN REDUCED MOTILITY AND INCREASED THE PERCENTAGE OF CELLS STAINING WITH EOSIN. DIFFERENCES BETWEEN COOLING RATES WERE NOT SIGNIFICANT.
Details
- Original title: COLD SHOCK AND ITS PREVENTION BY EGG YOLK IN SPERMATOZOA OF THE CAT.
- Record ID : 1986-0890
- Languages: English
- Source: Cryo-Letters - vol. 6 - n. 4
- Publication date: 1985
- Document available for consultation in the library of the IIR headquarters only.
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Sperm; Egg yolk; Cryoprotectant; Cooling rate; Cell; Cat; Survival
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