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COMPARISON OF STEM CELL VIABILITY OF BONE MARROW CRYOPRESERVED BY TWO DIFFERENT METHODS.

Author(s) : WANG S. Y.

Type of article: Article

Summary

TWO DIFFERENT METHODS WERE USED TO STUDY THE PRESERVATION OF MURINE BONE MARROW CELLS. COMPARED TO THE METHODS, IN WHICH SEPARATED MONONUCLEAR MARROW CELLS IN 10% DMSO WERE CRYOPRESERVED IN LIQUID NITROGEN, A MODIFIED TECHNIQUE WAS CARRIED OUT BY CRYOPRESERVATION OF UNFRACTIONATED MARROW CELLS IN A MIXED PROTECTANT OF 5% DMSO AND 6% HYDROXYETHYL STARCH (HES) AT 193 K (-80 DEG C). AFTER STORAGE FOR 1, 4, 8, AND 12 WEEKS SAMPLES WERE ASSAYED FOR CELL VIABILITY AND RECOVERY. THE DMSO/H ES PROTECTANT PROVIDES A SIMPLIFIED BONE MARROW CRYOPRESERVATION TECHNIQUE THAT SHOULD BE FAVORABLE TO CLINICAL APPLICATION BECAUSE OF ITS HIGH STEM CELL RECOVERY AND AVOIDANCE OF CELL-SEPARATION MANIPULATION.

Details

  • Original title: COMPARISON OF STEM CELL VIABILITY OF BONE MARROW CRYOPRESERVED BY TWO DIFFERENT METHODS.
  • Record ID : 1989-0350
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 3
  • Publication date: 1987

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