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RESPONSIVENESS OF MOUSE CALVARIA TO PARATHYROID HORMONE AFTER EXPLANT CRYOPRESERVATION: 45 CALCIUM RELEASE IN VITRO.

Author(s) : WEZEMAN F. H., DUNGAN D. D.

Type of article: Article

Summary

NEWBORN MOUSE CALVARIA PRELABELED WITH 45 CALCIUM AND CRYOPRESERVED AT 77 K (-196 DEG C) IN SERUM-FREE MEDIUM CONTAINING DMSO WERECOMPARED TO UNPRESERVED EXPLANTS FOR RESPONSE TO PARATHYROID HORMONE DURING SUBSEQUENT CULTURE. AFTER SHORT TERM CRYOPRESERVATION FOLLOWED BY RAPID THAWING, THE VIABLE EXPLANTS CONTINUED TO RELEASE 45 CALCIUM TO THE CULTURE MEDIUM BUT ADDITIONS OF PARATHYROID HORMONE TO THE MEDIUM DID NOT CAUSE INCREASED BONE RESORPTION. CRYOPRESERVATION AND THAWING IMPAIRS MECHANISMS RESPONSIBLE FOR PARATHYROID HORMONE ACTION ON BONE CELLS.

Details

  • Original title: RESPONSIVENESS OF MOUSE CALVARIA TO PARATHYROID HORMONE AFTER EXPLANT CRYOPRESERVATION: 45 CALCIUM RELEASE IN VITRO.
  • Record ID : 1987-0378
  • Languages: English
  • Source: Cryobiology - vol. 23 - n. 4
  • Publication date: 1986

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